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Evidence Guide: MSL974001 - Prepare, standardise and use solutions

Student: __________________________________________________

Signature: _________________________________________________

Tips for gathering evidence to demonstrate your skills

The important thing to remember when gathering evidence is that the more evidence the better - that is, the more evidence you gather to demonstrate your skills, the more confident an assessor can be that you have learned the skills not just at one point in time, but are continuing to apply and develop those skills (as opposed to just learning for the test!). Furthermore, one piece of evidence that you collect will not usualy demonstrate all the required criteria for a unit of competency, whereas multiple overlapping pieces of evidence will usually do the trick!

From the Wiki University

 

MSL974001 - Prepare, standardise and use solutions

What evidence can you provide to prove your understanding of each of the following citeria?

Prepare solutions

  1. Select appropriate procedure for solution preparation
  2. Select equipment, materials and solvent of specified purity
  3. Measure appropriate quantities of reagents for solution preparation and record data.
  4. Select and assemble specified laboratory equipment and appropriate grade of glassware
  5. Perform specified dilutions
  6. Prepare solutions to achieve homogeneous mix of the specified concentration
  7. Label and store solutions to maintain identity and stability
Select appropriate procedure for solution preparation

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Select equipment, materials and solvent of specified purity

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Measure appropriate quantities of reagents for solution preparation and record data.

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Select and assemble specified laboratory equipment and appropriate grade of glassware

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Perform specified dilutions

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Prepare solutions to achieve homogeneous mix of the specified concentration

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Label and store solutions to maintain identity and stability

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Standardise and use volumetric solutions

  1. Assemble appropriate laboratory equipment
  2. Perform serial dilutions as required
  3. Standardise the solution to the required specified range and precision
  4. Label and store solutions to maintain identity and stability
  5. Use standard volumetric solutions to determine concentration of unknown solutions
Assemble appropriate laboratory equipment

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Perform serial dilutions as required

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Standardise the solution to the required specified range and precision

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Label and store solutions to maintain identity and stability

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Use standard volumetric solutions to determine concentration of unknown solutions

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Calculate and record data

  1. Calculate specified concentrations
  2. Use authorised procedure if data is to be modified
  3. Estimate and document uncertainty of measurement in accordance with workplace procedures, as required
  4. Record all relevant details according to laboratory procedures and report results
  5. Report concentration with appropriate units
Calculate specified concentrations

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Use authorised procedure if data is to be modified

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Estimate and document uncertainty of measurement in accordance with workplace procedures, as required

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Record all relevant details according to laboratory procedures and report results

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Report concentration with appropriate units

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Monitor the quality of laboratory solutions

  1. Check solutions for visual deterioration and expiry date
  2. Re-standardise or dispose of dated or deteriorated solutions
  3. Record details and label solutions according to laboratory procedures
Check solutions for visual deterioration and expiry date

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Re-standardise or dispose of dated or deteriorated solutions

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Record details and label solutions according to laboratory procedures

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Maintain a safe work environment

  1. Use established safe work practices and personal protective equipment (PPE) to ensure personal safety and that of other laboratory personnel
  2. Clean up spills using appropriate techniques to protect personnel, work area and environment
  3. Minimise generation of waste and environmental impacts
  4. Ensure the safe collection of laboratory and hazardous waste for subsequent disposal
  5. Store equipment and reagents as required
Use established safe work practices and personal protective equipment (PPE) to ensure personal safety and that of other laboratory personnel

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Clean up spills using appropriate techniques to protect personnel, work area and environment

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Minimise generation of waste and environmental impacts

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Ensure the safe collection of laboratory and hazardous waste for subsequent disposal

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Store equipment and reagents as required

Completed
Date:

Teacher:		 Evidence:

 

 

 

 

 

 

 

Assessed

Teacher: ___________________________________ Date: _________

Signature: ________________________________________________

Comments:

 

 

 

 

 

 

 

 

Instructions to Assessors

Evidence Guide

Elements describe the essential outcomes.

Performance criteria describe the performance needed to demonstrate achievement of the element.

1

Prepare solutions

1.1

Select appropriate procedure for solution preparation

1.2

Select equipment, materials and solvent of specified purity

1.3

Measure appropriate quantities of reagents for solution preparation and record data.

1.4

Select and assemble specified laboratory equipment and appropriate grade of glassware

1.5

Perform specified dilutions

1.6

Prepare solutions to achieve homogeneous mix of the specified concentration

1.7

Label and store solutions to maintain identity and stability

2

Standardise and use volumetric solutions

2.1

Assemble appropriate laboratory equipment

2.2

Perform serial dilutions as required

2.3

Standardise the solution to the required specified range and precision

2.4

Label and store solutions to maintain identity and stability

2.5

Use standard volumetric solutions to determine concentration of unknown solutions

3

Calculate and record data

3.1

Calculate specified concentrations

3.2

Use authorised procedure if data is to be modified

3.3

Estimate and document uncertainty of measurement in accordance with workplace procedures, as required

3.4

Record all relevant details according to laboratory procedures and report results

3.5

Report concentration with appropriate units

4

Monitor the quality of laboratory solutions

4.1

Check solutions for visual deterioration and expiry date

4.2

Re-standardise or dispose of dated or deteriorated solutions

4.3

Record details and label solutions according to laboratory procedures

5

Maintain a safe work environment

5.1

Use established safe work practices and personal protective equipment (PPE) to ensure personal safety and that of other laboratory personnel

5.2

Clean up spills using appropriate techniques to protect personnel, work area and environment

5.3

Minimise generation of waste and environmental impacts

5.4

Ensure the safe collection of laboratory and hazardous waste for subsequent disposal

5.5

Store equipment and reagents as required

Required Skills and Knowledge

Elements describe the essential outcomes.

Performance criteria describe the performance needed to demonstrate achievement of the element.

1

Prepare solutions

1.1

Select appropriate procedure for solution preparation

1.2

Select equipment, materials and solvent of specified purity

1.3

Measure appropriate quantities of reagents for solution preparation and record data.

1.4

Select and assemble specified laboratory equipment and appropriate grade of glassware

1.5

Perform specified dilutions

1.6

Prepare solutions to achieve homogeneous mix of the specified concentration

1.7

Label and store solutions to maintain identity and stability

2

Standardise and use volumetric solutions

2.1

Assemble appropriate laboratory equipment

2.2

Perform serial dilutions as required

2.3

Standardise the solution to the required specified range and precision

2.4

Label and store solutions to maintain identity and stability

2.5

Use standard volumetric solutions to determine concentration of unknown solutions

3

Calculate and record data

3.1

Calculate specified concentrations

3.2

Use authorised procedure if data is to be modified

3.3

Estimate and document uncertainty of measurement in accordance with workplace procedures, as required

3.4

Record all relevant details according to laboratory procedures and report results

3.5

Report concentration with appropriate units

4

Monitor the quality of laboratory solutions

4.1

Check solutions for visual deterioration and expiry date

4.2

Re-standardise or dispose of dated or deteriorated solutions

4.3

Record details and label solutions according to laboratory procedures

5

Maintain a safe work environment

5.1

Use established safe work practices and personal protective equipment (PPE) to ensure personal safety and that of other laboratory personnel

5.2

Clean up spills using appropriate techniques to protect personnel, work area and environment

5.3

Minimise generation of waste and environmental impacts

5.4

Ensure the safe collection of laboratory and hazardous waste for subsequent disposal

5.5

Store equipment and reagents as required

Evidence of competence in this unit must satisfy all of the requirements of the elements and performance criteria, and include demonstration of:

preparing and standardising at least three (3) different solutions in accordance workplace procedures and/or standard methods

using balances and volumetric glassware accurately

selecting and using primary and secondary standards and indicators

performing titrations using laboratory procedures with required accuracy and precision and within required timelines

determining equivalence points using indicators and graphical methods

calculating the concentration of the solution with appropriate units and uncertainties given the chemical reaction for the titration

performing quality assurance checks for solution performance

recognising control results that are not within acceptable range

recording and reporting results in accordance with workplace procedures

labelling and storing solutions, safely cleaning up spills and collecting and disposing of waste in accordance with workplace procedures

working safely at all times by following established safe work practices and using safety information and safety equipment.

Must provide evidence that demonstrates knowledge of:

solution terminology

the chemistry of acids, bases, buffers and redox reactions

Moles and Molarity

scientific terminology for different types of solutions

chemical symbols and Atomic Mass

formulae for calculating concentration

concepts of metrology, including:

all measurements are estimates

measurements belong to a population of measurements of the measured parameters

precision, accuracy and significant figures

sources of error, uncertainty and repeatability

traceability

international system of units (SI)

grades of glassware, reagents and their use

reactions used for standardisation and desirable characteristics

workplace procedures, standard methods and calculations used for preparing, standardising and monitoring the quality of solutions used in job role

workplace communication and reporting procedures

relevant hazards, work health and safety (WHS) and environment requirements.

Range Statement

This field allows for different work environments and conditions that may affect performance. Essential operating conditions that may be present (depending on the work situation, needs of the candidate, accessibility of the item, and local industry and regional contexts) are included.

Standards, codes, procedures and/or workplace requirements

Standards, codes, procedures and/or workplace requirements include the latest version of one or more of:

Australian and international standards covering the requirements for the competence of testing and calibration laboratories, laboratory safety, quality management, laboratory glassware and international system of units (SI)

national work health and safety (WHS) standards and codes of practice, national environmental protection measures, and national measurement regulations and guidelines

specific codes, guidelines and procedures, such as National Association of Testing Authorities (NATA) accreditation requirements, principles of good laboratory practice (GLP), and Australian code of good manufacturing practice for medicinal products (GMP)

workplace documents, such as standard operating procedures (SOPs); quality and equipment manuals; calibration and maintenance schedules; material safety data sheets (MSDS) and safety procedures; material, production and product specifications; production and laboratory schedules; workplace recording and reporting procedures; and waste minimisation and safe disposal procedures

workplace procedures and standard methods for preparing working solutions

Solutions

Solutions may include, but are not limited to, one or more of:

solutions of strong/weak acids and bases

oxidising/reducing agents

solutions used for complexometric or precipitation titrations

stains for cells and tissues, enzymes, buffers and antibodies

diluents for maintaining isotonicity

organic solutions and histological fixatives

Checking useability of solutions

Checking useability of solutions includes, but is not limited to, one or more of:

examining stained samples for correct staining reactions

performing pH checks

confirming enzyme activity

checking red cell suspensions for haemolysis

ferric chloride for phenolic solutions

isotonicity for saline

Established safe work practices

Established safe work practices include, but are not limited to, one or more of:

use of PPE, such as safety glasses, gloves and coveralls

correctly labelling reagents and hazardous materials

handling and storing hazardous material and equipment in accordance with labels, MSDS, manufacturer instructions, and workplace procedures and regulations

regular cleaning and/or decontamination of equipment and work areas

prompt clean-up of spills in accordance workplace procedures

WHS and environmental management requirements

WHS and environmental management requirements

· complying with WHS and environmental management requirements at all times, which may be imposed through state/territory or federal legislation. These requirements must not be compromised at any time

· applying standard precautions relating to the potentially hazardous nature of samples

· accessing and applying current industry understanding of infection control issued by the National Health and Medical Research Council (NHMRC) and State and Territory Departments of Health, where relevant